Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Zambrano-Zaragoza, Jose Francisco; Duran-Avelar, Ma de Jesus; Messina-Robles, Maud; Vibanco-Perez, Norberto

dc.contributor.author	Zambrano-Zaragoza, Jose Francisco
dc.contributor.author	Duran-Avelar, Ma de Jesus
dc.contributor.author	Messina-Robles, Maud
dc.contributor.author	Vibanco-Perez, Norberto
dc.date.accessioned	2017-05-16T19:48:54Z
dc.date.available	2017-05-16T19:48:54Z
dc.date.issued	2012-06
dc.identifier	https://doi.org/10.4269/ajtmh.2012.11-0741	es_ES
dc.identifier.issn	0002-9637	es_ES
dc.identifier.uri	http://hdl.handle.net/123456789/946
dc.description	Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked freshwater fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific region of Mexico, and one of its etiologic agents has been identified as Gnathostoma binucleatum. We characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total IgM, IgE, and IgG class and subclasses against a crude extract of the parasite by Western blotting. Our results do not show differences in the antigens recognized by IgM and IgE. However, we found that the specific humoral immune response is caused mainly by IgG, specifically IgG4. We found that 43%, 65.2%, 54.1%, and 26.3% of the patients recognize the 37-kD, 33-kD, 31-kD, and 24-kDa antigens, suggesting that the 33-kD antigen is the immunodominant antigen of G. binucleatum.	es_ES
dc.description.abstract	La gnathostomiasis es una enfermedad parasitaria sistémica emergente adquirida al consumir peces de agua dulce crudos o no cocidos infectados con las larvas avanzadas de la tercera etapa de Gnathostoma spp. Esta enfermedad es endémica de la región del Pacífico de México, y uno de sus agentes etiológicos ha sido identificado como Gnathostoma binucleatum. Se caracterizó la respuesta inmune humoral de los pacientes clínicamente diagnosticados con gnathostomiasis mediante la detección de IgM total, IgE, IgG y subclases contra un extracto crudo del parásito por Western blot. Nuestros resultados no muestran diferencias en los antígenos reconocidos por IgM e IgE. Sin embargo, se encontró que la respuesta inmune humoral específica es causada principalmente por IgG, específicamente IgG4. Se encontró que el 43%, el 65,2%, el 54,1%, y el 26,3% de los pacientes reconocen el 37-kD, 33-kD, 31-kD, Y antıgenos de 24 kDa, lo que sugiere que el antıgeno de 33 kD es el antıgeno inmunodominante de G. binucleatum.	es_ES
dc.language.iso	spa	es_ES
dc.publisher	The American Journal of Tropical Medicine and Hygiene	es_ES
dc.relation.uri	Público en general	es_ES
dc.rights	info:eu-repo/semantics/openAccess	es_ES
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0	es_ES
dc.source	Zambrano-Zaragoza, J. F., de Jesús Durán-Avelar, M., Messina-Robles, M., & Vibanco-Pérez, N. (2012). Characterization of the humoral immune response against Gnathostoma binucleatum in patients clinically diagnosed with gnathostomiasis. The American journal of tropical medicine and hygiene, 86(6), 988-992.	es_ES
dc.subject	Humoral Immune	es_ES
dc.subject	Gnathostoma binucleatum	es_ES
dc.subject	Patients Clinically Diagnosed with Gnathostomiasis	es_ES
dc.subject.classification	MEDICINA Y CIENCIAS DE LA SALUD [3]	es_ES
dc.title	Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis	es_ES
dc.type	info:eu-repo/semantics/article	es_ES