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dc.contributor.author | RODRIGUEZ CERVANTES, CARLOS HUMBERTO | |
dc.contributor.author | GIRON PEREZ, MANUEL IVAN | |
dc.contributor.author | ROBLEDO MARENCO, MARIA DE LOURDES | |
dc.contributor.author | SANCHIS, V. | |
dc.contributor.author | MARIN, S. | |
dc.contributor.author | RAMOS, J. A. | |
dc.date.accessioned | 2017-05-01T23:27:25Z | |
dc.date.available | 2017-05-01T23:27:25Z | |
dc.date.issued | 2012-05-14 | |
dc.identifier.issn | 1465-3443 | es_ES |
dc.identifier.uri | http://dspace.uan.mx:8080/jspui/handle/123456789/807 | |
dc.description | A survey of fungal contamination and presence of aflatoxins (AFs) and fumonisins (FBs) in 30 feed samples collected from 10 tilapia farms during three seasons in Nayarit State, located in north-western Mexico, was carried out using ELISA as screening and High-Performance Liquid Chromatography (HPLC) as confirmatory method. Mycobiota included Aspergillus flavus and Fusarium spp. AFs were detected in 63.3% of samples using ELISA, but confirmation by HPLC revealed that all samples were under the detection limit. Regarding to FBs, positive samples were detected using both methods, with 19 positive samples (60% of total) by ELISA and 14 positive samples (46.6% of total) by HPLC and levels ranging from 0.148 to 2.587 mg/kg. Correlation was observed between both methods (r = 0.516, p = 0.004) for FBs results. No sample exceeded the European maximum levels for any of the mycotoxins. Water activity of samples ranged from 0.345 to 0.655, suggesting that mycotoxin occurrence is probably related to raw material contamination. | es_ES |
dc.description.abstract | Se llevó a cabo un estudio de contaminación por hongos y presencia de aflatoxinas (AF) y fumonisinas (FB) en 30 muestras de pienso de 10 granjas de tilapia durante tres temporadas en el estado de Nayarit, en el noroeste de México, Performance Liquid Chromatography (HPLC) como método de confirmación. Mycobiota incluyó Aspergillus flavus y Fusarium spp. AFs se detectaron en el 63,3% de las muestras utilizando ELISA, pero la confirmación por HPLC reveló que todas las muestras estaban bajo el límite de detección. En cuanto a FBs, se detectaron muestras positivas utilizando ambos métodos, con 19 muestras positivas (60% del total) por ELISA y 14 muestras positivas (46,6% del total) por HPLC y niveles de 0,148 a 2,587 mg / kg. Se observó correlación entre ambos métodos (r = 0,516, p = 0,004) para los resultados de FBs. Ninguna muestra superó los niveles máximos europeos para ninguna de las micotoxinas. La actividad de agua de las muestras varió de 0,345 a 0,655, lo que sugiere que la presencia de micotoxinas probablemente esté relacionada con la contaminación de la materia prima. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Food and Agricultural Immunology | es_ES |
dc.relation.uri | Público en general | es_ES |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0 | es_ES |
dc.source | http://www.tandfonline.com/doi/abs/10.1080/09540105.2012.684202 | es_ES |
dc.subject | feed | es_ES |
dc.subject | tilapia | es_ES |
dc.subject | mycobiota | es_ES |
dc.subject | ELISA | es_ES |
dc.subject | aflatoxins | es_ES |
dc.subject | fumonisins | es_ES |
dc.subject.classification | BIOLOGÍA Y QUÍMICA [2] | es_ES |
dc.title | DETERMINACION OF AFLATOXIN AND FUMONISIN LEVELS THROUGH ELISA AND HPLC, ON TILAPIA FEED IN NAYARIT, MEXICO | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |