CHARACTERIZATION OF THE HUMORAL IMMUNE RESPONSE AGAINST GNATHOSTOMA BINUCLEATUM IN PATIENTS CLINICALLY DIAGNOSED WITH GNATHOSTOMIASIS

ZAMBRANO ZARAGOZA, JOSE FRANCISCO; DURAN AVELAR, MARIA DE JESUS; MESSINA ROBLES, MAUD; VIBANCO PEREZ, NORBERTO

dc.contributor.author	ZAMBRANO ZARAGOZA, JOSE FRANCISCO
dc.contributor.author	DURAN AVELAR, MARIA DE JESUS
dc.contributor.author	MESSINA ROBLES, MAUD
dc.contributor.author	VIBANCO PEREZ, NORBERTO
dc.creator	76344	es_ES
dc.creator	21787	es_ES
dc.creator	77480	es_ES
dc.date.accessioned	2019-05-30T19:53:37Z
dc.date.available	2019-05-30T19:53:37Z
dc.date.issued	2012-06-01
dc.identifier	https://doi.org/10.4269/ajtmh.2012.11-0741	es_ES
dc.identifier.issn	1476-1645	es_ES
dc.identifier.uri	http://dspace.uan.mx:8080/jspui/handle/123456789/1982
dc.description	Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked freshwater fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific región of Mexico, and one of its etiologic agents has been identified as Gnathostoma binucleatum. We characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total IgM, IgE, and IgG class and subclasses against a crude extract of the parasite by Western blotting. Our results do not show differences in the antigens recognized by IgM and IgE. However, we found that the specific humoral immune response is caused mainly by IgG, specifically IgG4. We found that 43%, 65.2%, 54.1%, and 26.3% of the patients recognize the 37-kD, 33-kD, 31-kD, and 24-kDa antigens, suggesting that the 33-kD antigen is the immunodominant antigen of G. binucleatum.	es_ES
dc.description.abstract	La gnatostomiasis es una enfermedad parasitaria sistémica emergente adquirida por el consumo de pescado de agua dulce crudo o crudo infectado con larvas avanzadas de tercera etapa de Gnathostoma spp. esta enfermedad es endémica en la región del Pacífico de México, y uno de sus agentes etiológicos ha sido identificado como Gnathostoma binucleatum. Caracterizamos la respuesta inmunitaria humoral de los pacientes diagnosticados clínicamente con gnathostomiasis detectando las clases y subclases de IgM, IgE e IgG totales contra un extracto crudo del parásito por Western blotting. Nuestros resultados no muestran diferencias en los antígenos reconocidos por IgM e IgE. Sin embargo, encontramos que la respuesta inmunitaria humoral específica es causada principalmente por IgG, específicamente IgG4. Encontramos que el 43%, 65.2%, 54.1% y 26.3% de los pacientes reconocen los antígenos 37-kD, 33-kD, 31-kD y 24-kDa, sugiriendo que el antígeno 33-kD es el antígeno inmunodominante de G. binucleatum.	es_ES
dc.language.iso	eng	es_ES
dc.publisher	The American Society of Tropical Medicine and Hygiene	es_ES
dc.relation.ispartof	CONACYT
dc.relation.uri	Público en general	es_ES
dc.rights	info:eu-repo/semantics/openAccess	es_ES
dc.rights.uri	http://creativecommons.org/licenses/cc-by-nc-sa	es_ES
dc.source	http://www.ajtmh.org/docserver/fulltext/14761645/86/6/988.pdf?expires=1559243784&id=id&accname=guest&checksum=839E3D54B0CB35A7AC66BE31C487ABD7	es_ES
dc.subject	gnatostomiasis	es_ES
dc.subject	enfermedad parasitaria	es_ES
dc.subject	enfermedad endémica	es_ES
dc.subject	antígenos	es_ES
dc.subject	parasitic disease	es_ES
dc.subject	endemic disease	es_ES
dc.subject	antigens	es_ES
dc.subject.classification	MEDICINA Y CIENCIAS DE LA SALUD [3]	es_ES
dc.title	CHARACTERIZATION OF THE HUMORAL IMMUNE RESPONSE AGAINST GNATHOSTOMA BINUCLEATUM IN PATIENTS CLINICALLY DIAGNOSED WITH GNATHOSTOMIASIS	es_ES
dc.type	info:eu-repo/semantics/article	es_ES